Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1E10
- UniProt No.
- P37840
- NCBI Accession No.
- NP_000336
- Alternative names
- SNCA, NACP, PARK1, alpha-Synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Alpha synuclein, Alpha-synuclein isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, Non-A-beta component of alzheimers disease amyloid, precursor of PARK 1, PARK 4, PARK4, Parkinson disease familial 1, PD 1, PD1, Synuclein alpha,
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human alpha-synuclein (1-140aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of alpha-synuclein in LnCap cells. The cell was stained with ATGA0244 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of alpha-synuclein in HeLa cells. The cell was stained with ATGA0244 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant human synuclein family (alpha-, beta- and gamma-) proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha-synuclein antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: alpha-synuclein
Lane 2.: beta-synuclein
Lane 3.: gamma-synuclein
Lane 1.: alpha-synuclein
Lane 2.: beta-synuclein
Lane 3.: gamma-synuclein
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.