Human Cytokeratin 8 antibody

Cat No.
ATGA0253

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT3D11
UniProt No.
P05787
NCBI Accession No.
NP_002264
Alternative names
Keratin type II cytoskeletal 8, Keratin, type II cytoskeletal 8, keratin 8, CARD2, CK-8, CK8, CYK8, K2C8, K8, KO

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human KRT8 (1-483aa) purified from E. coli
Isotype
IgG3 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of KRT8 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of KRT8 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: LnCap cell lysate
Lane 2.: PC3 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: A549 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of KRT8 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.