NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT1G7

UniProt No.

Q07021

NCBI Accession No.

NP_001203

Alternative names

Complement C1q binding protein, Complement component 1, Q subcomponent binding protein, GC1QBP, Glycoprotein gC1Q-R, gC1qR, Mitochondrial matrix protein p32, ASF/SF2-associated protein p32, SF2p32, C1q globular domain-binding protein, Hyaluronan-binding protein 1, Splicing factor SF2-associated protein

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human C1QBP (74-282aa) purified from E.coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of C1QBP in HeLa cells. The cell was stained with ATGA0330 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant protein
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : 293T cell lysate
Lane 7 : A549 cell lysate