Human HABP1/C1QBP antibody

Cat No.
ATGA0330

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1G7
UniProt No.
Q07021
NCBI Accession No.
NP_001203
Alternative names
Complement component 1 q subcomponent binding protein, p33, HABP1, gC1qR, GC1QBP, SF2p32,Complement component 1 q subcomponent binding protein, C1QBP, Complement component 1, q subcomponent binding protein C1q globular domain binding protein, Complement component 1 Q subcomponent binding protein mitochondrial, GC1Q R, GC1q R protein, GC1QR, Glycoprotein gC1qBP, HABP 1, Hyaluronan binding protein 1, Mitochondrial matrix protein p32, p32, Pre mrna splicing factor SF2 P32 subunit precursor, Splicing factor SF2 associated protein.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human C1QBP (74-282aa) purified from E.coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of C1QBP in HeLa cells. The cell was stained with ATGA0330 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant protein
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : 293T cell lysate
Lane 7 : A549 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.