Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT3G7
- UniProt No.
- Q16543
- NCBI Accession No.
- NP_008996
- Alternative names
- Cell division cycle 37 homolog, P50CDC37, CDC37, Cell division cycle 37 homolog, Cell division cycle 37 homolog S cerevisiae hypothetical protein CDC37, CDC 37, CDC37 cell division cycle 37 homolog, Cdc37 homolog, CDC37 protein, Hsp90 chaperone protein kinase targeting subunit, Hsp90 chaperone protein kinase targeting subunit p50Cdc37, Hsp90 co chaperone Cdc37, p50, p50Cdc37.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human CDC37 (1-378aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of CDC37 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDC37 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDC37 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Jurkat cell lysate
Lane 2. : A431 cell lysate
Lane 3. : K562 cell lysate
Lane 4. : LnCap cell lysate
Lane 5. : SW480 cell lysate
Lane 6. : HeLa cell lysate
Lane 7. : MCF7 cell lysate
Lane 8. : 293T cell lysate
Lane 1. : Jurkat cell lysate
Lane 2. : A431 cell lysate
Lane 3. : K562 cell lysate
Lane 4. : LnCap cell lysate
Lane 5. : SW480 cell lysate
Lane 6. : HeLa cell lysate
Lane 7. : MCF7 cell lysate
Lane 8. : 293T cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.