Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT3B7
- UniProt No.
- Q9Y3D6
- NCBI Accession No.
- NP_057152
- Alternative names
- Tetratricopeptide repeat domain 11, FIS1, Tetratricopeptide repeat domain 11 2010003O14Rik, CGI 135, CGI135, FIS 1, Fis1 homolog, FIS1, S, cerevisiae, homolog of, Fission 1 (mitochondrial outer membrane) homolog, Fission 1 (mitochondrial outer membrane) homolog (S, cerevisiae), Fission 1 (mitochondrial outer membrane) homolog (yeast) Fission 1 homolog, H NH0132A01.6, hFis 1, hFis1, Mitochondrial fission 1 protein, Tetratricopeptide repeat protein 11, TPR repeat protein 11, TTC 11.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human TTC11 (1-122aa) purified from E. coli.
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of TTC11 in HaLa cells. The cell was stained with ATGA0490 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TTC11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: MCF7 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: MCF7 cell lysate
The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TTC11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: TTC11 transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: TTC11 transfected 293T cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.