Human Actin antibody

Cat No.
ATGA0517

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT2F5
UniProt No.
P68133
NCBI Accession No.
NP_001091
Alternative names
ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Actin (13-377aa) purified from E.coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Actin in HeLa cells. The cell was stained with ATGA0517 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Lung Tissue lysate
Lane 2.: HeLa cell lysate
Lane 3.: A549 cell lysate
Lane 4.: A431 cell lysate
Lane 5.: Raji cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.