Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT2F5
- UniProt No.
- P68133
- NCBI Accession No.
- NP_001091
- Alternative names
- ACTA, ACTA1, Actin, alpha 1, Alpha actin 1, Alpha skeletal muscle, Alpha skeletal muscle actin, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3, Skeletal muscle
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Actin (13-377aa) purified from E.coli
- Isotype
- IgG2a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of Actin in HeLa cells. The cell was stained with ATGA0517 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Lung Tissue lysate
Lane 2.: HeLa cell lysate
Lane 3.: A549 cell lysate
Lane 4.: A431 cell lysate
Lane 5.: Raji cell lysate
Lane 1.: Mouse Lung Tissue lysate
Lane 2.: HeLa cell lysate
Lane 3.: A549 cell lysate
Lane 4.: A431 cell lysate
Lane 5.: Raji cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.