NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

3A4

UniProt No.

P20339

NCBI Accession No.

NP_004153

Alternative names

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

Additional Information

This product was produced from tissue culture supernatant.

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Rab5a (1-215aa) purified from E. coli.

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ATGA0519 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Rab5a Transfected 293T cell lysate

Related Publications

Gorji-Bahri G, et al. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. (J Cell Biochem. 2021)
Gorji-Bahri G, et al. RAB5A effect on metastasis of hepatocellular carcinoma cell line via altering the pro-invasive content of exosomes. (Exp Mol Pathol. 2021)
Polla DL, et al. Biallelic variants in TMEM222 cause a new autosomal recessive neurodevelopmental disorder. (Genet Med. 2021)
Nanou A, et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis. (Cell Rep. 2021)
Huang HW, Ryoo HD. Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival. (PLoS Genet. 2021)
Patel M, et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. (Br J Cancer. 2022)
Tang Y, et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. (Acta Pharm Sin B. 2022)