Human Rab5a antibody

Cat No.
ATGA0519

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
3A4
UniProt No.
P20339
NCBI Accession No.
NP_004153
Alternative names
RAB5A member RAS oncogene family, RAB5A, member RAS oncogene family, RAB5, Rab5a, RAB5A, member RAS oncogene family RAB 5, RAB 5A, RAB5A member RAS oncogene family, RAS associated protein RAB5A, Ras related protein Rab 5A.
Additional Information
This product was produced from tissue culture supernatant.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Rab5a (1-215aa) purified from E. coli.
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ATGA0519 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The mouse tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.