Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- 3A4
- UniProt No.
- P20339
- NCBI Accession No.
- NP_004153
- Alternative names
- RAB5A member RAS oncogene family, RAB5A, member RAS oncogene family, RAB5, Rab5a, RAB5A, member RAS oncogene family RAB 5, RAB 5A, RAB5A member RAS oncogene family, RAS associated protein RAB5A, Ras related protein Rab 5A.
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Rab5a (1-215aa) purified from E. coli.
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ATGA0519 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Rab5a Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: Rab5a Transfected 293T cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.