Human FABP1/L-FABP antibody

Cat No.
ATGA0521

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
2G4
UniProt No.
P07148
NCBI Accession No.
NP_001434
Alternative names
Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver
Additional Information
This product was produced from tissue culture supernatant.

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FABP1/L-FABP (1-127aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of FABP1/L-FABP in Hep3B cells. The cell was stained with ATGA0521 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1/L-FABP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human FABP1/L-FABP protein
Lane 2.: HepG2 cell lysate
Lane 3.: Mouse Liver tissue lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.