Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- 2G4
- UniProt No.
- P07148
- NCBI Accession No.
- NP_001434
- Alternative names
- Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human FABP1/L-FABP (1-127aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of FABP1/L-FABP in Hep3B cells. The cell was stained with ATGA0521 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1/L-FABP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human FABP1/L-FABP protein
Lane 2.: HepG2 cell lysate
Lane 3.: Mouse Liver tissue lysate
Lane 1.: Recombinant human FABP1/L-FABP protein
Lane 2.: HepG2 cell lysate
Lane 3.: Mouse Liver tissue lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.