Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- 3E5
- UniProt No.
- P05161
- NCBI Accession No.
- NP_005092
- Alternative names
- Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP,IFI15,ISG15,Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene,15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDaprotein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, InterferonStimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier,ubiquitin cross reactive protein precursor
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human ISG15 (1-157aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of ISG15 in HeLa cells. The cell was stained with ATGA0543 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: MCF7 cell lysate
Lane 2.: PC3 cell lysate
Lane 1.: MCF7 cell lysate
Lane 2.: PC3 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.