Human Cyclophilin B antibody

Cat No.
ATGA0545

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
k2E2
UniProt No.
P23284
NCBI Accession No.
NP_000933
Alternative names
PPIB, Peptidylprolyl isomerase B, CYPB, SCYLP, CYP-S1: Cyclophilin-B, Cyclophilin B, 26- 216aa, AA553318, AI844835, Cphn 2, Cphn2, CyP 20b, AA408962, CYP S1, MGC14109, MGC2224, peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, PPIase, Rotamase, S cyclophilin
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Cyclophilin B (26-216aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ATGA0545 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: Jurkate cell lysate
Lane 4.: K562 cell lysate
Lane 5.: 293T cell lysate
Lane 6.: A549 cell lysate
Lane 7.: MCF7 cell lysate
Lane 8.: sk-ov-3 cell lysate
Lane 9.: LnCap cell lysate
The Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human PPIB Protein
Lane 2.: Recombinant human PPIA Protein
Lane 3.: Recombinant human PPID Protein
Lane 4.: Recombinant human PPIE Protein
Lane 5.: Recombinant human PPIF Protein
Lane 6.: Recombinant human PPIG Protein
Lane 7.: Recombinant human PPIH Protein

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.