Human NM23-H1 antibody

Cat No.
ATGA0585

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT5F4
UniProt No.
P15531
NCBI Accession No.
NP_000260
Alternative names
Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1, Non-metastatic cells 1 AWD, drosophila, homolog of, NDPKA, NM23, NM23-M1, Granzyme A activated DNase, GZMA activated DNase, Metastasis inhibition factor nm23, NDK A, NDP Kinase A, NM23 long variant, included, Nonmetastatic protein 23, NME1-NME2 spliced read-through transcript, included, Non-metastatic cells 1, protein (NM23A) expressed in, Nonmetastatic cells 1, protein expressed in, NME1, Nucleoside diphosphate kinase A, Nonmetastatic protein 23, homolog 1, NM23H1B, included, Tumor metastatic process-associated protein
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human NME1 (1-152aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of NM23-H1 in HeLa cells. The cell was stained with ATGA0585 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of NM23-H1 in A549 cells. The cell was stained with ATGA0585 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human NME1 protein
Lane 2.: Recombinant human NME2 protein
Lane 3.: Recombinant human NME3 protein
Lane 4.: Recombinant human NME4 protein
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: PC3 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.