Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT5F4
- UniProt No.
- P15531
- NCBI Accession No.
- NP_000260
- Alternative names
- NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human NME1 (1-152aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of NM23-H1 in HeLa cells. The cell was stained with ATGA0585 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of NM23-H1 in A549 cells. The cell was stained with ATGA0585 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human NME1 protein
Lane 2.: Recombinant human NME2 protein
Lane 3.: Recombinant human NME3 protein
Lane 4.: Recombinant human NME4 protein
Lane 1.: Recombinant human NME1 protein
Lane 2.: Recombinant human NME2 protein
Lane 3.: Recombinant human NME3 protein
Lane 4.: Recombinant human NME4 protein
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: PC3 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: A549 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: PC3 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.