Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT41E2
- UniProt No.
- P11233
- NCBI Accession No.
- NP_005393
- Alternative names
- Ras-related protein Ral-A, RAL,Ras-related protein Ral-B
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human RALA (1-203aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, FACS
- Usage
- The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human RalA protein
Lane 2.: Recombinant human RalB protein
Lane 1.: Recombinant human RalA protein
Lane 2.: Recombinant human RalB protein
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: NIH3T3 cell lysate
Lane 4.: PC3 cell lysate
Lane 5.: U87MG cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: NIH3T3 cell lysate
Lane 4.: PC3 cell lysate
Lane 5.: U87MG cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained with ATGA0590 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.