NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

3F3

UniProt No.

Q9NS23

NCBI Accession No.

NP_009113

Alternative names

RASSF1(Ras association (RalGDS/AF-6) domain family 1),123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association (RalGDS/AF-6) domain family member 1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human RASSF1A (1-340aa) purified from E. coli.

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

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ICC/IF analysis of RASSF1A in HeLa cells. The cell was stained with ARF0501 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

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ICC/IF analysis of RASSF1A in A431 cells. The cell was stained with ARF0501 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate

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Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: RASSF1A transfected 293T cell lysate

Related Publications

Gou HF, et al. Epidermal growth factor receptor (EGFR)-RAS signaling pathway in penile squamous cell carcinoma. PLoS One. 2013 Apr 24;8(4):e62175.
Korah R, et al. Epigenetic silencing of RASSF1A deregulates cytoskeleton and promotes malignant behavior of adrenocortical carcinoma. Mol Cancer. 2013 Aug 5;12:87.
Beckedorff FC, et al. The intronic long noncoding RNA ANRASSF1 recruits PRC2 to the RASSF1A promoter, reducing the expression of RASSF1A and increasing cell proliferation. PLoS Genet. 2013;9(8):e1003705.
Thaler S, et al. RASSF1A inhibits estrogen receptor alpha expression and estrogen-independent signalling: implications for breast cancer development. Oncogene. 2012 Nov 22;31(47):4912-22.
Guo C, et al. The tumor suppressor RASSF1A prevents dephosphorylation of the mammalian STE20-like kinases MST1 and MST2. J Biol Chem. 2011 Feb 25;286(8):6253-61.
Thaler S, et al. RASSF1A mediates p21Cip1/Waf1-dependent cell cycle arrest and senescence through modulation of the Raf-MEK-ERK pathway and inhibition of Akt. Cancer Res. 2009 Mar 1;69(5):1748-57.
Bennett KL, et al. AP-2alpha induces epigenetic silencing of tumor suppressive genes and microsatellite instability in head and neck squamous cell carcinoma. PLoS One. 2009 Sep 9;4(9):e6931.
Liu L, et al. RASSF1A interacts with and activates the mitotic kinase Aurora-A. Oncogene. 2008 Oct 16;27(47):6175-86.
Wang X, et al. Id-1 promotes chromosomal instability through modification of APC/C activity during mitosis in response to microtubule disruption. Oncogene. 2008 Jul 24;27(32):4456-66.
Chiu RW, et al. Hypermethylation of RASSF1A in human and rhesus placentas. Am J Pathol. 2007 Mar;170(3):941-50.