3T3-L1 Lysate

Cat No.
ATGL0021

Size

QTY

List Price

100ug

USD 80


200ug

USD 110

500ug

USD 200

USD 0



Product Information

Product Type
Cell Lysate

Product Specification

Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Storage
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
General Description
3T3-L1 cell line was derived from mouse 3T3 cells that is used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype. 3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. These cells are also sensitive to lipogenic and lipolytic hormones and drugs, including epinephrine, isoproterenol, and insulin. 3T3-L1 cells are extensively used to study adipogenesis.
Quality Control
Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis (WB)

All lanes : 3T3-L1 Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.